For the double-40, each experiment knocked-down two genes and the number of experiments was double the number of genes (m = 2 n = 40 ).
In response to this comment we performed an additional experiment, knocking down FMN-2 and then quantifying nuclear oxidation in response to MMS treatment.
The reverse experiment knocking down β-catenin via shRNA blocked the BMP-6-induced proliferation of LNCaP and 22Rv1cells in RPMI-1640/1% cFBS.
In these experiments, knock down of ERK1 did not significantly affected IM action, supporting the critical role for ERK2.
Expression of the receptor in filamin-deficient M2 cells together with siRNA experiments knocking down FLNa in HEK293 cells, demonstrated that lack of FLNa delays the internalization of the receptor.
In line with the above data, in our experiments knocking down Hv1 expression in leukemia cell lines PLB-985 and Jurkat, resulted in reduced IHv density (Figs. 5 and S3, respectively).
Control experiments knocking down PI5P4Kα and β did not show the same effect on dome formation.
The scuffle leads to their experiment getting knocked over, staining the carpet and filling the room with smoke.
Then, in vitro studies, we took serum starvation-refeeding experiment and knocked down the expression of SOX4 with siRNA to demonstrate that SOX4 could promote proliferation of NPC nonkeratinizing cell line CNE2.
In a mirror series of experiments, we knocked down RARα in the retinoid-sensitive HER2+/ER− and Luminal SKBR3 cells by stable transfection of a RARα1/3-targeting shRNA.
To test for functional relevance, we performed epistasis experiments and knocked down Esrrb, Klf2, Tbx3, and other pluripotency-associated transcription factors including Nanog in Tfe3-ERT2-expressing cells.
