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Usually, a quasi-static experiment is required leading to small compression force rates.
Nevertheless, the voltage cannot be increased again because the external voltage of the AFM used in the experiment is required to be no more than 10 V.
If siRNA-mediated downregulation of a specific gene provides a detectable cellular phenotype, a rescue experiment is required to see whether co-expressing the targeted gene with the siRNA restores the wildtype phenotype.
For each disease, a set of candidate genes (e.g., from a linkage study) has to be defined and a relevant microarray experiment is required (e.g., diseased sample vs. reference sample).
An additional PCR experiment is required to rule out that the absence of an amplification product is due to a technical artifact: the breakpoint forward primer is combined with a "human specific" reverse primer (confirmation_primers), designed in the corresponding non-disrupted region of the human chromosome (Figure 1B).
This implies that in order to actually distinguish between these two, a different experiment is required.
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Further testing with real-life airbags and comparison with experiments is required.
It is also a time consuming process because a large number of experiments is required.
However to establish this assumption additional experiments are required.
Phantom experiments are required to confirm these assumptions.
Totally, 20 experiments were required for this work.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com