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Data were combined from replicate samples and grouped in experiment interpretations.
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Fourth, in addition to being incomplete, available functional annotations are error prone because of experiment interpretation or curation issues37,46.
In this study particular care was given to MTSET/MTS-2-MTS reactivity experiment interpretation, as "run-ups" attributed to de-phosphorylation of the channel by endogenous patch-associated phosphatases are known to occur in CNGA1 CNGA1els48.
MT supervised, conceived and designed the experiment, interpretation of the data and revision of the manuscript.
DP involved in the design of the experiment, interpretation of data and manuscript review.
WM conceived the study, assisted in designing the experiment, interpretation of the data, and finalizing the manuscript.
JG conceived the study, participated in the design of the experiment, interpretation of the data, and helped to draft the manuscript.
In addition, controls (n = 16 movies) were set to 1.00 with related group results scaled proportionally to facilitate inter-experiment interpretations.
CS: performing experiments, interpretation.
MWFC: performing experiments, interpretation.
KF: performing experiments, interpretation.
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