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During the experiment, heart rate and arterial pressure were unchanged.
At the end of the experiment, heart was perfused with PBS followed by 4%% paraformaldehyde at the time of sacrifice to remove blood and for initial fixation.
Throughout the entire experiment heart rate was measured in each subject as an approximation to his state of alertness across the different sessions of rest (Pre1, Pre2, and Post).
During the experiment, heart rate was recorded continuously by means of a three-lead ECG.
Towards the end of the experiment, heart rate did not differ among groups (Table 1).
During the whole experiment heart rate was significantly higher in ZL rats than in untreated and metformin-treated ZDF rats.
Similar(51)
At the end of each experiment, hearts were rapidly freeze-clamped with tongs cooled in liquid nitrogen and stored frozen at −80°C for later determination of enzyme activity.
At the end of each experiment, hearts were dissected and the different compartments weighed.
After the experiment, hearts were isolated to rinse with ice-cold phosphate-buffered saline (PBS) and weighed.
At the end of each experiment, hearts were removed from the perfusion system and the atria and large vessels cut away.
At the end of experiment, hearts of each group were dissected out for Western blotting analysis and Real-time reverse transcription-polymerase chain reaction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com