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Minimum of 50% of the surviving An. culicifacies mosquitoes from each feeding experiment, fed on the same blood isolate were dissected on day '9' and '10' in normal saline (0.65% NaCl) and stained in a drop of 0.5% mercurochrome [38].
The rats were fasted for 12 h prior to experiment, fed at 4 h later dosing, and water was accessed ad libitum throughout the study period.
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On the basis of above experiments, fed-batch cultivation of A. utahensis ZJB-08196 coupled with exogenous addition of validamine in fermentation medium was further investigated.
In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls.
Further study on experiments of fed-batch process demonstrated the use of complex, non-differentiable model and produced improved process performances using the optimal feeding profile.
Subsequent experiments with fed-batch shaking flasks demonstrated the feasibility of improving antibody production using a biphasic addition strategy for H&T: supplementing a low concentration of H&T during initial cell growth phase and a high concentration of H&T at the production phase.
For the fed-batch experiment, compressed air was fed into the bioreactor through a gas flowmeter (Manostat 125, New York, NY, USA), which was manually adjusted to a flow rate of 100 mL/min.
A field experiment on rain-fed spring wheat (Triticum aestivum) was conducted at the Haiyuan Experimental Station (36°34′N, 105°39′E), in a semiarid region of China, during 2001 2003.
At present, there is no report on the specific effect of Pb-induced oxidative stress in a pair-fed experiment.
Temperature range was set to 20 30°C and pH from 5 to 3 according to experiment and the fed-batch phase (glycerol feeding) was prolonged, whereas the induction time was shortened.
In experiment 1, rats fed RS had more ACF than that of the control fed rats.
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