What started as an experiment dehydrating fruit in his mother's kitchen has grown into a partnership with Hattie Larlham, an Ohio nonprofit that serves children and adults with developmental disabilities, to use their shared commercial kitchen space and workforce to craft Peaceful Fruits' snack bars.
And when he experiments with dehydrating fruits and vegetables, the obligatory slow speed and 104-degree temperature of the process yields humor along with health benefits.
Prior to experiments, the hydrogels were dehydrated by incubation in an ethanol series with increasing concentrations of ethanol (50% (v/v), 60% (v/v), 70% (v/v), 80% (v/v), 90% (v/v), 2 × 100% (v/v)) in water for 10 min each.
For the histological study, after two days of fixation, parasaggital sections were taken through the hilus from 4 lung lobes per time point in two separate experiments, and these were dehydrated and embedded in paraffin, sectioned at 5 µM and stained with haematoxylin and eosin.
To perform this experiment, fresh brain tissues were dehydrated by replacing the water with paraffin.
In one ingenious experiment, Oettingen had participants rendered mildly dehydrated.
For immunohistochemistry experiments embryos or heads of newborn mice were dehydrated, embedded in paraffin, and cut into 10 µm serial sections.
Very good resistance of the smooth YSZ products to the corrosion in highly dehydrated Li/Na molten carbonates has been shown in experiments lasting few 1000 h.
To understand the effect of hydration on protein dynamics, inelastic neutron-scattering experiments were performed on staphylococcal nuclease samples at differing hydration levels: dehydrated, partially hydrated, and hydrated.
The ∼1 kPa polyacrylamide hydrogel was dehydrated at 4°C overnight and was rehydrated immediately before the AFM experiment with a standard phosphate buffered saline (PBS) solution.
For in situ hybridization experiments, embryos were fixed for 2 h in 4% MEM-PFA, dehydrated in an ethanol series and stored in 75% ethanol at −20 °C as described68,70.
