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The multiple-dose RIPC group received one dose of RIPC per day on the 6 days before the endotoxemia experiment day, and 1 dose just before LPS administration.
We always read the optical density (OD) at the start of the experiment (Day 5) and the following days as indicated in Figure 9A.
Subjects taking prescription medication or experiencing clinically significant illness within 14 days before the endotoxemia experiment day were not allowed to participate.
Numbers of shoots and leaves per plant were counted at the start of the experiment (Day 0) and after 52 days when the aboveground biomass was harvested.
From day 172 (7 days after the initiation of nickel supplementation) until the end of the experiment (day 191), the methanol was fully removed without any VFA accumulation.
Day 6 was the endotoxemia experiment day.
At the end of the experiment (day 42) columns were dissected into three 8 cm sections.
Urokinase solutions were prepared in the morning and stored at 4 °C until being used on the experiment day.
In contrast, age-matched Hdac6 transgenic mice maintained body weight by the end of experiment day 12 (Fig. 2C).
On the experiment day, all mice were fasted for 6 hours and given an intraperitoneal injection of 2H2O.
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In the experiment, day-14 CAFCs and day-28 CAFCs revealed long-term suppression after irradiation, although CFUs-G/M did not show noticeable changes.
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