For all miRNA quantification experiments, cycle threshold (Ct) values greater than 36 were excluded.
Statistical analysis was performed using Student's t-test for the qRT-PCR experiments, cycle analyses, and proliferation, apoptosis and transcription assays.
Pure-breeding ancestors are used for replication within experiments (cycles) and genotypic concurrency across experiments (cycles).
For the semi-quantitative experiments, 40 cycles, 25 cycles and 35 cycles were performed for TRPC6, β-actin and CK19 respectively.
Many challenges remain in order to support the complete experiment life cycle in a federated environment.
It handles the complete experiment life cycle from instrument calibration, data acquisition, and real time data analysis to results publication.
The results of repetitive experiment and cycle experiment show that the g-C3N4@TiO2 has a strong binding force between the core and shell, which is stable, without secondary pollution and convenient for recovery.
During the experiment, day/night cycle was manipulated using specific wavelengths/colours.
In this study, 10 successive cycles were registered for each experiment: 5 cycles of wetting/dewetting for protein adsorption and few seconds just after and directly 5 cycles of wetting/dewetting in PBS solution for rinsing (desorption test).
In London, we have recently been experimenting with cycle couriers as well – it makes sense and it's better for the environment.
