For siRNA experiments, custom designed siRNA duplexes for RIP140, NCoR1 and SRC1 were purchased (Dharmacon, Lafayette, CO).
For DAX-1 gene silencing experiments, custom synthesized siRNA (Ambion) annealed duplexes were used for effective depletion of DAX-1 mRNA.
In summary, the experimental procedures used in this study consist, on the one hand, of real-time functional exploration sessions either with or without visual feedback to the subject and, on the other hand, of controlled follow-up experiments custom-designed to systematically test prominent reports of correlation between the ongoing power variations and the patient's behavior.
Beyond that, Balboni expects to experiment with "custom reporting"–an on-demand service where clients can hire GNE correspondents to generate custom news reports.
The experiment used custom software running on the PC, which was written in C++ and OpenGL 3.3.
ROIs defined in the localizer task were probed in the slow event-related experiment using custom software programmed in Matlab (The Mathworks Inc, Natick, MA).
In this experiment, a custom made phantom is imaged by AO-SDOCT.
The honeybee microarrays used for this experiment were custom spotted long oligonucleotide microarrays available from the University of Illinois (NCBI GEO Platform GPL15631 (13,440 double spotted oligos representing 13,135 sequences)).
To assess genome-wide changes in methylation patterns for each sample across a given experiment, a custom parameter termed the global methylation indicator (GMI) was calculated as previously described [ 8].
Cells used for single-cell CE experiments were plated the day before the experiments onto custom chambers, prepared by using poly(dimethylsiloxane) (PDMS, Sylguard 184) to glue a silicon O-ring (McMaster-Carr) to a #1 glass coverslip (Fisher).
