Over the time course of the experiment, currents expressed by scn8aa alone or scn8aa coexpressed with Scn1b inactivated fully.
Based on recent experiments, current approaches for designing ECB connections are examined, and a new strength characterization method is presented.
In these experiments, current steps were used instead of current ramps.
For EC50 experiments, current changes were measured for a range of PBA concentrations (1 10 mM).
In these experiments current was injected in one of the cells while the response to the current injection was recorded in the respective other cell [40].
During our experiments, current pulse amplitude was kept constant, which lead to a slightly higher voltage pulse amplitude when the neurons were under the influence of carbachol.
In voltage-clamp experiments, current amplitude (pA) was divided by the cell capacitance (pF) to give current density, pA/pF.
For these experiments, currents were filtered at 5 kHz and access resistance was compensated 80 90%.
For charge carrier experiments, currents were measured in modified KD50 containing either 50 mM KOH, RbCl, or CsCl.
For all experiments, currents were filtered at 1 kHz, digitized at 20 kHz, and analysed with pCLAMP9.2 and ORIGIN6.0 software.
