It is assumed that in the conditions used in a standard transgenesis experiment, construct molecules are interspersed with the carrier fragments in the concatemer and that the spacing between each construct molecules depends on the molar ratio carrier: construct [12].
If experiential learning activities support engagement and deeper student learning, student-owned experiments constructed "from the ground up" might have benefits that exceed pre-designed classroom experiences.
AP helped design yeast experiments, constructed strains and performed viability analysis and helped write the manuscript.
JMG conceived the study, designed experiments, constructed yeast strains, analysed data and wrote the paper.
YW designed the experiments, constructed the plasmids, performed transfections, and wrote the manuscript.
TSa designed the experiments, constructed the plasmids used in the study, performed experiments, drafted the manuscript, and edited the manuscript.
MG designed the study, performed the experiments, constructed RL-SAGE library, conducted data mining, and wrote the article.
CAH designed cellular and molecular experiments, constructed the SAGE libraries, carried out in vivo and in vitro experiments, and drafted the manuscript.
designed the project, performed all the molecular biology experiments, constructed the phylogenetic tree, analysed the qPCR data (Fig. 2) and wrote the manuscript.
WFM conceived and designed the experiments, constructed strains, conducted fluctuation tests to measure LOH rate, and conducted analyses of live cells during division.
