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In this experiment, compounds 2e and 3a significantly raised the amount of glucose uptake by the tissue both in the absence and presence of external insulin.
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In tubulin kinetics experiment, compound 6a destabilized polymerisation process (IC50 = 4.68 μM).
Moreover, in the vivo experiment, compound 6a showed comparable antibacterial activity to that of tiamulin, with ED50 of 5.47 mg/kg body weight against MRSA.
For the phosphate starvation experiment, compound plants were transferred into perlite and watered with half-strength Hoagland medium [ 75].
In laboratory experiments, compounds based on similar cube-shaped molecules have shown some ability to bind to cancer cells and destroy them.
In test tube experiments, compounds that bind to these heavy metals dissolve the plaques.
In all experiments, compounds were used in concentrations below the minimal inhibitory concentration.
For replication experiments, compounds were cherry-picked from thawed DMSO stocks, spotted manually onto assay plates and processed as above.
In these experiments, compounds are assayed in serial blood samples in the minutes following either feeding or injection of a solution containing the compounds.
According to the NMR data (ligand-observed as well as protein-observed experiments), compounds D1, D2 and D3 all bind to the PRDX5 protein, in the same region as the initial fragments.
Before used to the biological experiments, compounds were purified by re-crystallization or silica gel chromatography to reach the purity higher than 97.0%.
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CEO of Professional Science Editing for Scientists @ prosciediting.com