In the hemophilia experiment, cells were taken from the patients, and genes to make a key blood clotting factor were inserted into the cells.
In this experiment, cells were starved for 12 h before palmitate treatment.
Before the transport experiment, cells were washed three times with warm HBSS (pH 7.4, 37 °C).
In our experiment, cells always remained their spherical shapes while they were forming their cellular interaction with each other.
In one experiment, cells were injected into rats that had been treated with a chemical that destroyed spinal cord myelin.
For reversible experiment, cells were first imaged and incubated under ischemia.
Prior to each experiment, cells were incubated in respective serum free media for 24 hrs.
At the end of the experiment, cells were trypsinized and counted to assess the adherent population under these conditions.
Forty eight hrs before the experiment, cells were split into MEM supplemented with FBS and antibiotics but without G418.
During the experiment, cells were grown and visualized in 12-well coverslip bottom plates (MatTek) coated with fibronectin (Sigma).
Before each experiment, cells were starved for 24 hours by serum deprivation (Phenol red-free RPMI containing 1% L-Glutamine).
