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A screening experiment by using a Plackett-Burman design was first performed in order to determine the main contributing factors to the electrochemical response.
Vanderweyde stated the spring balance used in Crookes' experiment was unreliable as it was easy to manipulate by deception and suggested he should repeat the experiment by using a chemical balance.
The body temperature of all subjects was maintained within physiological range (37±0.8°C) throughout the experiment, by using a water heating system incorporated in the stereotactic holder.
The core temperature was maintained at 38.0 ± 0.5°C during the experiment by using a heating pad.
Colitis was evaluated on the last day of the experiment by using a disease activity index (DAI), colon length, and histological scoring system.
The assay was slightly modified for this experiment by using a larger reaction volume (2 mL total volume), which accommodates multiple sampling over time.
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A negative control was performed for each experiment by using an unrelated primary antibody.
The calibration curve was prepared at the maximum ofloxacin absorption peak of 293.8 nm before the experiment by using an ultraviolet-visible spectrophotometer (Lambda950, Perkinelmer, USA).
We conducted experiments by using a 20-L nearly spherical gas explosion testing device.
These conditions have been obtained in experiments by using a lithium-coated vessel interior, optimized gas fuelling (also assisted by pellet injection) and plasma shapes that minimize plasma wall interactions.
The concentration of para-H2 (in comparison to orthohydrogen) was determined before the experiments by using a home-made temperature-stabilized thermal-conductivity cell based on a heated tungsten filament47,48,49,50.
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