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It was once widely seen as an experiment bound to fail.
"But it seems to me, this is an experiment bound to fail". .
Gurtner et al. tried to stably overexpress NF-YA in cells, an experiment bound to failure, including in our hands.
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Orbital ATK's Antares rocket was carrying a Cygnus spacecraft loaded with more than 5,000 pounds of supplies, equipment and scientific experiments bound for the International Space Station. .
On Sunday morning, SpaceX successfully launched and landed a Falcon 9 rocket carrying a Dragon spacecraft loaded with over 5000 pounds of supplies and experiments bound for the International Space Station.
In one set of experiments, bound GST-Vt was then further incubated with 10-fold molar excess (5 µM) of phosphatidylinositol-4,5-bisphoshpate (PIP2) at 37°C for 1 hr.
In contrast, the allosteric MEK inhibitors used in these experiments bind to a MEK-specific pocket that is distinct from the ATP-binding pocket, and freeze the enzyme in the inactive conformation (Ohren et al., 2004).
Of the remaining 46 peptides, 34 did not bind in any experiment, 9 bound in 1 of 6 experiments, and 3 bound in 2 of 6 experiments.
Recently, we developed an experimental assay and analysis pipeline that allows measurement of binding energies between a single TF and up to 106 DNA species in a single experiment (Binding Energy Topography by sequencing, or BET-seq) (Le et al., 2018).
Of the remaining 52 peptides, 43 did not bind in any experiment, 7 bound in 1 of 6 and 2 bound in 2 of 6 experiments.
The experiment was bound by a self-imposed rule: no personal electronics like iPods, portable DVD players or laptops.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com