He proposed a new experiment: animals would be trained to associate the tone with the electric shock.
At the end of each experiment, animals were euthanized by rapid injection of Euthasol®.
At the end of the experiment, animals were sacrificed through exsanguination.
After the imaging experiment, animals should be monitored during awakening from anaesthesia.
At the terminal experiment animals were 6.1 ± 0.2 months of age and considered to be young-adult (Table 1).
At the end of the experiment, animals were killed with an additional dose of pentobarbital and a KCl bolus.
At the end of the experiment, animals were euthanized with an additional dose of pentobarbital and a potassium chloride bolus.
At the end of the experiment, animals were euthanized by rapid injection of Euthasol® (Virbac Corporation, Fort Worth, TX, USA).
In the seventh week of the experiment animals were sacrificed, blood was collected for plasma and uteri were removed.
Throughout the experiment, animals were housed in two chambers, in which the diet was offered four times daily.
At the start of the experiment, animals were scanned using [18F]JNJ42259152 prior to any treatment to asses baseline BPND values.
