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We performed an 8-week experiment, a time frame generally endorsed in other uremic models of renal failure to study CKD complications such as vascular calcification [ 10].
Finally, the Boyden chamber is also limited because cell counting requires termination of the experiment; a time course therefore requires multiple devices.
The bleach region was a diffraction-limited spot, and the bleach time was 20 ms. For each FRAP experiment, a time series of 120 images of a fixed confocal plane was acquired every 60 ms, while the bleach pulse was aimed and triggered manually by mouse click.
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Figure 8 Experiment A: SNR time series for PRN 25.
In each experiment a time-sequence of fluorescence images was acquired with one frame taken every 345 ± 10 ms with 250 ms exposure time.
In a previous cortical photo-injury experiment, a time-course study of nestin demonstrated expression at 4 days, peaking at 7 days and becoming undetectable by 30 days following injury (Suzuki et al., 2012), paralleling observations in other experimental injury models (Frisen et al., 1995; Krum & Rosenstein, 1999; Shibuya et al., 2002; Suzuki et al., 2012).
In concordance with the knockdown experiments, a time course assay showed no significant proliferative differences between the CD133+/CD133− populations.
With the starting amount of cells kept equal across experiments, a time dependent decrease of the total amount of isolated total RNA was obtained.
We assessed timing in two different experiments: a time-estimation (TE) and a time-discrimination (TD) task.
Transforming development economics, one experiment at a time.
The second type of experiment is a time course.
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