Indeed, in microarray-based experiments, a lower hybridization can result either from a reduced amount of transcripts or from divergence in the sequence of the gene [20].
For in vivo experiments, a lower resolution lens (40 μm 1/e spot at the focus for the OCT imaging beam) was used for an increased depth of focus and less divergent photothermal beam.
For UPS experiments, a lower nominal film thickness of 10 nm was chosen, which is, on the one hand, sufficiently low to avoid sample charging issues and, on the other hand, large enough to avoid an influence of the HOPG substrate on the PFP electronic structure.
This is similar to what was found by Campo et al. (2003) for the ADP ribose- and depolarization-activated conductances in macrophages (although in our experiments a lower lanthanum concentration was more effective), but disagrees with the finding of Kraft et al. (2004) that La3+ did not block the ADP ribose-activated current in cultured rat microglia.
To gain further insight into the kinetics of sII hydrate formation, we performed a third experiment at a lower pressure, 100 bar, and 252 K.
This value is comprehensible considering the primary power of 2.3 kW used for this experiment, since a lower value was expected due to different losses in the plasma jet system and only a fraction of the total energy is transferred to the surface.
Moreover in dogs an orally administered dose of 40 mg/kg 24 times at regular intervals for 60 days caused necrotic liver cell death while the same experiment at a lower dose of the tetrandrine (20 mg/kg) caused reversible injury to liver cells.
Therefore, we believe that peak forces in our experiment represent a lower boundary for estimates of motor copy number.
It had been previously reported that cloning experiments would reveal PCR Taq DNA polymerase errors at the rate of 1 1000 bases [ 61], although the GoTaq DNA polymerase (Promega) used in this experiment had a lower error rate [ 62].
Moreover, rat 6 refused to work on the task at one point, forcing us to terminate this part of the experiment at a lower number of sampled trials than intended (cf. Table 1).
