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Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography.
I was afraid this might turn out to be a very expensive column.
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A disadvantage is a requirement for large volumes of purified solvent (low-pressure or gravity systems) or expensive columns (HPLC).
It is tailored to our protocol of plasmid isolation that uses centrifugations steps instead of expensive columns.
Furthermore, the use of non-chromatographic affinity tags could eliminate the need for expensive columns, and may also be easy to scale up.
As a unique feature the robot comprises the integration of a centrifuge instead of expensive columns, the use of a custom-made pipetting head with a movable gripper, especially designed shaking platforms and an acetone wash facility.
The advantage of this method proposed over previously reported is that the enantio-recognition can be performed in a high efficiency without requiring an expensive chiral column.
Additionally, the method avoids the use of expensive chiral columns and more sophisticated instrumentation, as it is the case of discrimination using liquid chromatography alternatives.
These huprines have been prepared by the above-mentioned four-step sequence from the readily available bicyclo[3.3.1]nonane-3,7-dione, which involves two rather expensive silica gel column chromatography purifications.
Furthermore, it was found that a crude filtered toluene extract caused irreparable clogging of the very expensive specialized HPLC columns that were used (Cosmosil BuckyPrep; the formation of unstable soluble TMF silver complexes was thought to be responsible).
Since these columns are expensive in terms of cost and energy consumption, an accurate determination of their separation efficiency is a prerequisite to optimization of their performance by design modification and revamping.
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CEO of Professional Science Editing for Scientists @ prosciediting.com