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The use of multiple restriction enzymes for TMDH has theoretical advantages, but increases the expense of the method since it involves an additional set of microarrays.
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Additionally, a multiresolution technique is an option to lower the computational expense of the presented method.
It is unfortunate that the expense of the best method renders it unavailable in RLS.
We found that the expense of the new method is typically less than half of the cost of the traditional technique of individually labeling each primer prior to nonmultiplexed PCR reactions (Appendix S1).
The method is named the 'local level set method', since it localizes the level set method near the interface to significantly reduce the computational expense of the level set method.
The generation of random genomes under different models greatly facilitates the study of a wide range of genomic features in relation to the underlying compositional biases, and the flexibility of the approach is only limited by the computational expense of the associated Monte Carlo method.
The increased expense of such methods and the diminishing number of skilled labourers capable of dressing the granite have led to a search for alternatives.
The presence of multiple local maxima or minima in the objective function drove the scientists to develop evolutionary algorithms as the genetic algorithm (GA) at the expense of conventional methods, such as gradient-based algorithm.
However, the expense of AIMD methods limits their applicability to systems on the order of 100 atoms and picosecond time scales.
It is noted that the main computational expense of the algorithm is the Lattice Boltzmann method.
However, this comes at the expense of using MCMC methods that are computationally intensive, but these methods have the advantage that computing time and memory requirements increase linearly with the number of observations and number of markers.
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