Exact(6)
To ensure that expression divergence signified by low interspecies correlations was outside the range of expected variation between members of the same species, we computed the intraspecies correlations of the 760 P. falciparum genes utilized in our initial analysis using microarray studies of the 3D7, Dd2 and HB3 strains of P. falciparum [18].
The expected variation between lanes for that exon also changes.
Because these city-specific estimates were not heterogeneous, this may reflect the expected variation between independent studies.
Further, there was expected variation between the size of the homogenized pieces that were between 10 μm and 500 μm (data not shown).
On average only about 12% of the total probesets on the GeneChip disagreed between any two comparisons that we made, which is well within the range of expected variation between samples in any DNA microarray study (data not shown).
Thus, it is important to further evaluate the technology, addressing questions concerning expected variation between closely related strains, background variation, and SNP variation that may arise during sub-culturing possibly obscuring an accurate molecular epidemiological analysis of isolates associated with contamination or outbreak events.
Similar(54)
This technology produces results comparable to those of central laboratory assays with only minimal, expected variations between sites (23).
This study demonstrates that the current nature of the technology is to expect variation between single lanes both in exon detection and estimation of gene expression using RPKM.
This represents an expected variation of between 0.9% and -1.5% compared with a 6.0% growth forecasted in August.
In each model, the distances between OTUs were modified according to the expected variation in gene flow between OTUs (see Methods section).
As expected, variation in lobular composition between individuals was observed (Additional file 4: Figure S2a).
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