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Hence, as well as inducing general toxic-response pathways, there will also be specific biological mechanisms for copper handling that may be expected to be perturbed, and thus copper is an excellent model toxin for demonstrating an integrative ecotoxicogenomics approach.
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The NBD fluorophore exploited to determine lipid distribution between the two bilayer leaflets is small, and expected to be minimally perturbing.
Somite markers (specifically somites 20-25) arexpecteded to be present in the wild type and perturbed in spt in the 21-somite-stage tissue.
ROS may already perturb the intracellular environment during the experiment and thus can be expected to be a serious source of cell damage.
As we expected, the complexity of the redesigned TRNs, in terms of number of necessary TF to be perturbed, correlated with the amount of change induced by each viral infection to the transcriptome of infected plants.
There are genuine reasons to be perturbed at this sort of wolf-in-sheep's-clothing scenario.
(B, C) Transcriptional information of all TFs proposed to be perturbed in the designs.
To exit from pluripotency, the ground state must be perturbed.
As expected, many biological processes, such as metabolic process and responsive to stimulus, were perturbed under both chilling conditions since 446 DE genes were common to both CCA and CS (Additional file 2: Table S3A and Figure 3A).
We reasoned that charge reversal mutants (E16K and E20K) would be expected to significantly perturb binding if these residues play a key role and that reducing the bulk of F17 might weaken hydrophobic interactions during binding.
This renders NR2F6 an exceptional drug target because therapeutic intervention would not be expected to perturb the generic regulatory programs that are shared by other immune cell types.
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