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In SCC showing moderate to well differentiation, CD44 was expressed much more strongly in the basal compared to maturing tumor cells (Fig. 5B), consistent with the expected stem cell niches.
In contrast, the percentage of SP cells is much lower and within the range of expected stem cell frequency.
(B – C ) Expected stem cell clonal (red circles) distribution near a regional boundary (green line) in the absence (B ) or presence (C ) of stem cell regional autonomy.
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Radiation completely prevented pharynx regeneration in 100% of animals (n = 100 animals) indicating that, as expected, stem cells are required for regeneration.
In principle one would expect stem cell biologists to take the next few years to compare the usefulness of cells from each source -- if they have the funding.
While certain microenvironments in Drosophila, as expected, repress stem cell differentiation and promote adherence to the niche [ 8], even age-related differences in ECM composition directly influence stem cell function [ 32].
We have stably knocked down BRCA1 in MCF10A cells which, as expected, increased stem cell numbers in comparison with control cells as assessed by mammosphere formation and Aldefluor postivity.
"Millions of patients and their families expect that stem cell research will move forward as rapidly as possible," said Senator Edward M. Kennedy, Democrat of Massachusetts, the chairman of the Committee on Health, Education, Labor and Pensions, which held the hearing.
A feature that may be expected in stem cells is resistance to apoptosis following exogenous stress.
As expected for stem cells, rx2+ cells in the post-embryonic CMZ barely incorporate the thymidine analog bromodeoxyuridine (BrdU) when applied in short pulses (Supplementary Fig S2A) and similarly, there is only partial overlap with proliferation markers like phospho-histone H3 (pHH3) and proliferating cell nuclear antigen (PCNA) (Supplementary Fig S2B and C).
Cairns reasoned that there may be less cancer than might be expected because stem cells divide less frequently and are fewer in number, but he went further by making the radical proposal that they also protect one of their DNA strands during semi-conservative replication.
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