Given these concerns regarding the expected specificity of this measure, and the importance of high specificity when evaluating rare exposures (Dosemeci and Stewart 1996), further analyses of this measure were judged as unlikely to be informative and thus not performed.
It is estimated from the expected sensitivity of 0.99, expected specificity of 0.94, prevalence of oesophageal intubation as 0.15, with desired precision of 0.05 and confidence level of 95% based on research done by Chou H-C [14].
As expected, specificity increased for higher temperature pulse and pulse centiles, but at the expense of very poor sensitivity.
Western blotting of the H3K4 methylation antibodies against mono-, di- or tri-methylated H3K4 or monomethylated H3K9 synthetic peptides was performed and confirmed their expected specificity (Additional file 1, Figure S1).
The prediction confidence is based on the SVM output and computed as (1 – s), where s is the expected sensitivity for positive predictions or the expected specificity for negative predictions if the SVM output is used as the threshold in the ROC analysis.
The Mb preparations used for this study retain the ability to efficiently differentiate into Mt, and the Mb had the expected specificity of their expression profiles, indicating that they largely maintain their in vivo characteristics.
The simultaneous use of helper and competitor oligonucleotides with Clep1156 probe allowed the expected specificity without disturbing in situ accessibility.
Hidden Markov Models (HMMs) describing known GT signatures were collected from SUPERFAMILY (http://supfam.cs.bris.ac.uk/SUPERFAMILY/), CAZy (http://www.cazy.org/) and Pfam (http://pfam.sanger.ac.uk/) and subdivided into three groups depending on their expected specificity for GTs (Table 1).
These 12 HMMs were subdivided into three groups depending on their expected specificity for GTs, referred to as respectively I) 'Rossmann-fold domains', II) 'Sugar transferase' and 'UDP-Glycosyltranferase' and III) a set of nine more GT-specific HMMs.
This analysis revealed that only a portion of the single cell outgrowths was of expected specificity.
