Sentence examples for expected sequences are from inspiring English sources

These results are summarized in Figure  5 and Additional files 3 and 4. The expected sequences are reported and the identified peptides are highlighted or underlined.

On sequencing the recovered PCR products, the expected sequences were obtained.

Computational Analysis of Small RNA High-Throughput Sequencing Data Data processing and analyses were performed using custom Perl scripts and the statistical programming environment R. Fastq sequence reads with missing bases or barcodes not matching any of the expected sequences were excluded (if applicable).

Data processing and analyses were performed using custom Perl scripts and the statistical programming environment R. Fastq sequence reads with missing bases or barcodes not matching any of the expected sequences were excluded (if applicable).

While deviations from the expected sequence are observed they are restricted to point mutations and small indels which can be attributed to the fidelity of the polymerases used for the PCR amplification.

In both cases, plasmids possessing the expected sequence were purified using the QIAprep spin miniprep kit (Qiagen #27106) from 3 5 mL LB cultures grown for 16 20 h at 37°C.

Clones from which the expected sequence was confirmed were transferred to the pCB302-3 vectors.

After PCR amplification using sunflower genomic DNA as template, products with the expected sequence were obtained for 10/19 loci.

For this reason, these results are summarized in Figure  7, in which the expected sequence is reported and the identified peptides are underlined.

Primer and expected amplicon sequences are listed in Additional file 25.

A comparison of ESTs from different strains reveals that, as expected, coding sequences are less polymorphic than the 3' UTRs.