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A complete concordance was observed; all expected sequence variants that were detected using v3.6.2 were detected using v4.0.2 (17 SNVs, two insertions, and one deletion) (Supplementary Table 4, samples 4 9).
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Using the structure of the accepted wild type form of ovine PrP, the ALRQ allelic variant, an expected sequence substitution pattern for each amino acid position, given its structural environment, can be derived from the tables of the log-odds probability values.
As expected, all sequence variants in which mutations were previously detected yielded melting curves with abnormal shapes relative to wild-type DNA.
As would be expected, the frequency of sequence variants was higher in non-coding regions (one every 38 bp) than in coding regions (one every 86 bp).
The proposed whole-genome sequencing in the 1,000 Bull Genomes Consortium Project is expected to provide annotated sequence variants and genotypes of key ancestor bulls, which will enhance the quality of the genome enormously.
For these reasons, wild populations are expected to carry many novel nucleotide sequence variants and functional adaptations that are not present in domesticates.
The expected effect sizes of individual sequence variants on preeclampsia are small, but this approach is likely to decipher new clues to the pathogenesis.
It is expected that the abundance of sequence variants per OTU will decrease according to a power law if the variants are generated stochastically.
In four cell lines and the set of 28 tumours without paired normal tissue, a total of 81 sequence variants were expected.
This was suggested by Cohen et al (7), as they observed that protection against coronary heart disease risk was greater than expected by the effect of PCSK9 sequence variants on LDL cholesterol [6].
Since individual isolates or strains of T. cruzi are thought to evolve without significant between-strain genetic exchange due to the relative absence of sexual recombination [ 1], the accumulation of sequence variants would be expected.
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