As expected, γ2 was readily detectable by Western blotting in the WT and RG cells, using either anti-γ2 or anti-FLAG antibodies.
The investigation, using the Earth-orbiting Hubble Space Telescope, was keyed to look for sodium, a readily detectable element that astronomers expected to be present.
As shown in Figure 2H, biotinylation of proteins of intact cells showed readily detectable ICAM-2 proteins with the expected electrophoretic mobility for all cell lines.
TPD52 was readily detectable in SKBr3 cells (Figure S5A), as expected based on the increased gene copy number reported in this cell line [61].
Recombinant protein expression could hence be expected to be lower than that of HsAQP5 but should still be readily detectable with a western blot, unless there are obstructions in the translation that could contribute to the poor AtSIP1 1 expression.
As expected, the MAP1LC3-interacting sequestosome 1/p62 (SQSTM1), a cargo-recognition protein for autophagy was readily detectable in control SMGs of Atg5 KO, but not Atg5 WT mice.
Membrane-enriched fractions contained readily detectable levels of ATP1A1 and also ICAM-2 proteins of the expected molecular mass using an antibody that recognizes the CD domain of ICAM-2 (sc-1512; Santa Cruz Biotech).
This was readily detectable by the classic monoclonal pan-p63 antibody 4A4 that detects all isoforms.
However, the results of solid-state nuclear magnetic resonance on fresh-frozen and ground whole bones of several mammalian species revealed that the bone crystal OH− was readily detectable; a rough estimate yielded an OH− content of human cortical bone of about 20%% of the amount expected in stoichiometric HA (Cho et al. 2003).
As expected, Rho1 serves as an effective positive control for image-based assays, as treatment with Rho1 dsRNA results in a binuclear phenotype readily detectable using a DNA dye and any marker that defines the cell body.
