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Expected orientations are further supported by non-destructive magnetic methods.
The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations.
The acceptability of the expected orientations is confirmed by analyzing the velocity fields of the fluid throughout successive time-steps of the simulation and by linking those fields to the bending strengths (pre- and post-cracking) of prismatic specimens extracted from the wall.
If two reads of a pair are not mapped to the reference genome with the expected orientations and mapped distance, the pair is called a discordant pair and is likely to be caused by SVs [ 12- 14].
Several genes strongly associated with PKD such as Clusterin [ 55, 56], Vimentin [ 56], Timp [ 56], Bcl2 [ 57], several members of MAPK signaling [ 58] etc. were strongly differentially regulated in expected orientations, showing the reliability of mRNA expression profiles.
Our analysis shows that ~95% of the bovine CHORI-240 library clones with both a BAC fingerprint and two BESs mapping to the genome in the expected orientations (~27% of all BACs) have consistent locations in the BAC fingerprint map and the genome assembly.
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This is the expected orientation for γ iPP branching from α seeds.
The focal mechanisms of the large events represent normal faulting for which the expected orientation of the maximum compressive stress axis is nearly vertical (Fig. 1(b)).
In terms of orientation of BESs where both BESs were located on same chromosome, 63,888 clones had the expected orientation (BESs pointing toward each other; Figure 3C).
Inversions were predicted from paired BESs that pointed in either the opposition or same direction, as opposed to the expected orientation of toward each other.
The expected orientation of the stereocilia of the basilar papilla hair cells were taken as being at 90° to the abneural edge of the basilar papilla.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com