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For all H3-L61X strains used for subsequent experiments, sequencing of the corresponding OAD476-OAD477−amplified frevealed revealed the expected mutations at the H3-L61X codon and no additional mutations.
Even though the exons that encode this domain are not enriched for mutations over what was expected, mutations at hotspots R250, R400, and R412 in these exons account for a significant portion (12%) of the mutations in families with VWS/PPS.
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If in fact the domestications of the indica and japonica subspecies were completely independent, we might expect mutations at the same locus, but would not expect to see the same functional polymorphisms at domestication loci.
All SNaPshot assays identified the expected mutations.
As expected, mutation of Pias3 at Cys459 substantially diminished its S-nitrosation (Fig S11).
Even at an expected mutation concentration of less than 1%, the single color assay could accurately quantify the mutant.
Mutations were detected at a frequency similar to the expected mutation rates in the ovarian and colorectal cancer cohorts (Supplementary Table 5), considering the limited sample size.
Would you expect mutations that would also be buffered this way at the protein level?
The resulting composition in the act1 fadoubleble mutant line grown at 17°C indicates the profile expected for mutations at each of these steps.
The assumption that non-synonymous substitutions occur at the same rate as synonymous substitutions is as expected for mutations at the DNA level [ 29] and there is little or no evidence that nucleotide substitutions that would result in amino acid changes in the encoded protein occur at different rates than those which would not result in amino acid changes.
We used the software BOTTLENECK [21] to compare deviations from the observed heterozygosity at each locus in each L. leucozonium population with that expected at mutation-drift equilibrium.
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