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In activated B cells, HP1β showed the expected localisation to pericentromeric heterochromatin clusters.
Examination of sections labelled for TDP43 showed the expected localisation to euchromatin regions within nuclei, as previously described in normal human brain neurons (Thorpe et al., 2008).
Two had an expected localisation within the cytoplasma (KRT7) or the nucleus (CDK4), and two with no reports on protein localisation in urothelium (SDC1 and JUND).
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For example, one might expect localisation to cause the World Food Programme to shrink its size and influence in favour of local actors.
As expected, nuclear localisation was maintained in the wt protein and was rescued for the KNRQ and 8/K mutant proteins containing a TAg NLS (Fig. 4B, C).
Consistent with the expected intracellular localisation, Eag1 was detected at the cell membrane and in the cytoplasm, whereas HIF-1α expression was observed inside the nucleus and in the cytoplasm.
As expected, proper subcellular localisation in tubular structures (albeit with different efficiencies for the individual variants) was required for microtubule protection for all analysed variants (Table 1).
The results obtained are presented in Fig. 2D and show that the NLS mutations in PB2 lead to a much stronger phenotype than that expected from the intracellular localisation of the protein.
Twist showed both cytoplasmic and nuclear localisation as expected, considering its function as a transcription factor.
Comparison of our linkage map and the RH-based comparative map from INRA indicated a high correlation with the expected pattern of gene localisation.
These experiments showed expected abnormalities in chromosomal localisation at the centromere and mitotic spindles, together with endoreduplication associated with inhibition of cytokinesis.
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