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In a parallel analysis, Sf9 cells were also negative for TNCLV, as expected (lane 2).
No binding was observed in extracts from parental 293 cells as expected (lane 2 and 6).
An unrelated negative control antibody at the highest concentration (α-TvTIM2r) had no effect on RPC formation, as expected (lane 6).
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An excess of unlabeled unrelated transcripts was used as competitor RNA [ 19], which did not affect RPC formation, as expected, lanes 7, 8 and 15, 16, resp).
When Pol ε was added, it took over the leading strand and extended it full length; however, lagging strand Okazaki fragments were inhibited, a result we did not expect (lane 4).
As expected, HeLa (lane 11), 293T (lane 12) and SW1116 (lane 13) cells showed annexin I message.
Regarding the amount of sequencing that can be expected per lane of the HiSeq 2500, we have obtained similar results to standard whole genome sequencing on some libraries.
Regarding lane-keeping and driving speed, we expected a worse lane behavior and lower driving speed for older drivers than younger drivers.
The rice enzyme panel produced just over two hundred million 2 × 75 bp paired-end reads when run on an Illumina HiSeq 2500 (rapid mode) lane, which was approximately 33% above what would be expected from a lane of WGS sequencing per Illumina literature.
As expected HeLa cells (lane 1) expressed high levels of annexin I, and adenoids (lane 13) expressed annexin I, but at a much reduced level when compared to HeLa cells.
Maldonado did something very few in the pit lane expected him to - he showed immense maturity from beginning to end.
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CEO of Professional Science Editing for Scientists @ prosciediting.com