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ISH results showed the expected expression in the gonad for five of the six ovary-expressed genes and eight of the ten testis-expressed genes analyzed (see Table S7), indicating an 81.3% concurrence with our array data.
Higher than expected expression in N0 cancers, reflected also in higher expression in disease stage II than III, may result from failing to detect metastatic lymph nodes or presence of micrometastases and hence misclassification of some cancers.
How would this graph look for each individual strain aneuploidy/euploid comparison? Figure 4A and newly added Figure 4B show genes with lower-than, higher-than, or as expected expression in the combined group of aneuploid strains versus euploid references.
In the second step, genes were put in order by using a score list that was based on the number of publications mentioning the disease-gene association, relation to other diseases and expected expression in PBMC according to previous studies and our pilot experiment in which we analyzed selected genes in PBMC samples through individual RT-QPCR assays.
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However, we note that using their alternative approach of defining eQTLs, Nicolae et al [17] found that the enrichment was present across a number of different phenotype classes, even those in which you would not expect expression in the lymphoblast cell lines to play a role in the disorder.
Finally, the predicted target genes of the majority candidates present expected expression change in another independent data set, which serves as a supplementary validation of these candidates.
As expected, expression of CLE209/210 in stems was specific to developing phloem, with very low, if any, expression in xylem cells.
As expected, expression of TNFα in the synovium gradually increased during the course of the disease process.
As expected, expression of snR30 m2 in NOY504-pTH25 rm1 cells failed to rescue 18S rm1 expression (lane 6) and cell growth on galactose at 37oC.
As expected, expression of Gαo[Q205L] in the A nk2 −/− NMJs failed to restore synaptic stability when evaluated at the level of Synapsin or Futsch (supplementary material Fig. S3E,G,H).
As expected, expression of LZAP resulted in decreased p38 phosphorylation (Fig. 5B, top panel compare lanes 1 and 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com