Unlike the adult rat, during the postnatal period NPY is transiently expressed in several hypothalamic regions, along with the expected expression within the arcuate nucleus (ARH).
Expected expression levels of ASCL1 and NEUROD1 were confirmed by quantitative PCR (qPCR; Supplementary Fig. 5b, c).
As expected, expression of the ECM catabolic enzymes MMP-3, 9, 13 and ADAMTS-4 and 5 were strongly increased (Figures 4a f).
As expected, expression of Park2 alone had little effect on the fluorescence of mitochondria-localized GFP whereas further addition of CCCP reduced the GFP fluorescence by 56% (Fig. 2b,c).
To correct for length bias in comparisons of nuclei and whole-cell measurements, the nuclear gene expression levels were generated using featureCount28 (FPM values) and were normalized by the expected expression magnitude, as estimated by a generalized additive model.
As expected, expression levels varied between the two different constructs of each receptor.
As expected, expression of human ATL1 efficiently restored the ER network in double-depleted cells (Fig. 3A, 3C, and 3D).
Cre-mediated reversion of targeted insertion was also evaluated in 293T cells containing established targeted insertion at transcriptional start sites and expected expression reversion was observed.
As expected, expression of these genes was reactivated (Figure 6).
As expected, expression of saeR was abolished in 923Δsae.
As expected, expression of diurnally regulated genes was massively affected during cold acclimation.
