An available site is expected when the procrystal electron density is below a given threshold.
Therefore, the lower S4 values measured by BKK compared to the other stations can be understood in terms of the lower background electron density expected above the BKK station, which is located beneath the equatorial ionization anomaly trough.
For the ligand benzimidazole, as expected, we observed consistent electron density for the ligand at both temperatures for the primary binding site.[ 11] We were surprised to observe ligand density at three additional distal sites.
Some changes ~ 5 8% are expected in the ion and electron density due to this uncertainty in the model.
Note that the electron density is expected to be high between 1100 h IST and 1400 h IST when mesospheric echoes are observed more frequently.
As expected, the quality of the electron density map in these stretches is much better for monomer A than B. Monomer A is rotated nearly through 180° with respect to monomer B but due to the differences in local structure and B-factors between the two monomers, the two monomers are not related by a crystallographic two fold axis as in case of BsuL.
As expected from the deglycosylation process, electron density for a single N-acetylglucosamine-linked asparagine is clearly defined for residues N117, N186, N230, and N298 in the structure obtained at pH 5 where N298 has not been mutated.
However, for the N1s spectrum, the reverse phenomenon is expected because in N atoms, the electron density rises over the creation of the hydrogen bonds.
Such higher electron density could be expected with the involvement of lone pair of electron present on N-heterocycle (for example piperidine) with the carbon of pyridine ring to make a pi bond (one sigma and a pi bond formation results in a double bond between these atoms).
The Pb-density (red line) and the total e -density (blue -density the core meet quite closely the expected values for Pblueand the elinerof densitheof the shell that for core respectively.
