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Benchmarking on reference-free metagenome datasets would have resulted in a set of duplicate clusters and an expected duplication rate but would give no indication of the accuracy of each method for duplicate detection.
As expected, duplication of some ZmARFs showed a conserved intron/exon structure, whereas some others were more divergent, suggesting the possibility of functional diversification for these genes.
We defined genes with increased copy number as those with a log2 aCGH ratio greater than 0.7 (because relative intensity values are often slightly compressed from the expected duplication log2 value of 1.0); genes with a log2 aCGH ratio < −1.0 were identified as potentially deleted.
The expected duplication frequency of segmental and tandem duplication events in each family was calculated as follows: For tandem counts, a simulation was carried out, placing N genes of size 1 kb (where N is the size of the corresponding gene family) in a genome of approximately 100000 kb and counting how many pairs of genes were within a 50 kb window.
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As expected, duplications of universal domains positively correlated with duplications of universal protein families (r = 0.96, p = 4.50E-10), as did their deletions (r = 0.93, p = 8.35E-8 8.35E-8
As expected, the duplication of bi-lobe and the bi-lobe-associated Golgi was inhibited.
We also expected sequence duplication in the current analysis since mapped ESTs appear in several dense clusters on the same genetic positions in Figure 2 and seven of these ESTs were used to select multiple BAC clones.
As expected, the duplication rate decreased to 14.4% and read results were comparable to the depleted L. lactis library (Additional file 1: Table S4 and Additional file 2: Figure S10).
No families fall below one standard deviation below the median in both the segmental and tandem categories, but several are close: the mitochondrial carrier proteins and the heat shock transcription factor families have 9%and13%3% of the expected segmental duplications, respectively.
The second reason we expect accidental duplication not to be made unlawful derives from the economic rationale for copyright protection, which is to prevent free-riding on the authors expression.
Hence, although the de novo and the duplication-divergence model are both compatible with this pattern, one could have expected for a duplication model that more local clusters could have become apparent.
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