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We found that the H. magnipapillata gene groups with FoxO genes as expected (data not shown).
When the reporter constructs were expressed alone in E. coli, all but the positive control resulted in very low whole-cell fluorescence intensities, as expected (data not shown).
However, the occurrences of non-consensus tetranucleotides in this library were considerably less than the occurrences of consensus tetranucleotides as expected (data not shown).
Western analysis of the same whole cell lysates showed that ectopic Nab2 completely abrogated TGF-β-induced stimulation of Type I collagen synthesis, as expected (data not shown).
In all experiments, the type strain B. cereus DSM 31 (corresponding to ATCC 14579) reacted positive as expected (data not shown).
The resulting Bax/Bak deficient macrophages resisted staurosporine-induced ΔΨm dissipation as expected (data not shown) but were fully susceptible to Mtb-induced ΔΨm dissipation (Fig. 2D).
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This is true only if first BLAST hits are taken into account since increasing their number lowers performance, as one might expect (data not shown).
Although a precise congruence between the calculated data and the expected data is not obtained, total deviation of 3 °C at the peak can be considered acceptable.
However, unfortunately, the expected data were not obtained by examining the mapping pattern of RNA sequencing data under our experimental conditions.
As expected, data were not available for some patients because of the TMA core loss during processing, a well-recognised occurrence, therefore, data for all four antigens were available for only 282 patients.
Contrary to what might have been expected, data collection was not a rapid and straightforward process.
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