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As expected, complex tissues (brains, antennae and ovaries), show the highest level of transcriptional activity by expressing 60-70% genes, whereas a highly specialised organ (HP gland) expresses only 14% of genes.
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In view of the small fold changes expected from complex tissues such as brain, an extensive biomathematical workup including RMA-based normalization, fitting with a linear model, statistical ANOVA-based evaluation, stringent filtering, and representation of individual transcript changes per brain region in a decision matrix were applied to suppress background and to reveal the consistent effects.
Expect complex, spicy, sherried character.
Growing these complex tissues is much more challenging than growing sheets of skin or connective tissue.
Yet, he said, he still could not explain how cells organise themselves into such complex tissues.
Lewis's approach is only one of many in the wider effort to create complex tissues.
They have found that embryonic stem cells grown in this way can spontaneously organise themselves to form the complex tissues that make up eyes, glands and brain tissue.
"Tissue engineering to make simpler tissues has been a reality for a number of years and through what we have been able to achieve, we can start talking about larger, more complex tissues that are able to survive longer".
Redmond's contribution lay in bioprinting with living cells, and making complex tissues instead of simple sheets.
What started as a quest to understand brain development inadvertently grew into a potentially useful way to grow complex tissues in the lab.
"There seems to be no compelling reason why viable solid organs should be treated differently from less complex tissues on moral grounds," the council's report says.
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CEO of Professional Science Editing for Scientists @ prosciediting.com