Sentence examples for expected band of from inspiring English sources
An expected band of 730 bp was amplified from the four strains of A. tumefaciens, whereas no corresponding DNA band could be amplified from the 10 transformants.
A total of 10 randomly selected transformants were used for PCR verification using the specific primers designed for the hph gene, and the results revealed that an expected band of 500 bp was amplified from all of the 10 transformants.
Furthermore, Δtri3-2, Δtri3-6, Δtri3-10 and Δtri3-13 (lanes 16, 17, 17, and 21, respectively) showed the expected band of 3.6 kb (reduced by approximately 0.7 kb).
Agarose gel electrophoresis for the digested DNA showed the two linearized DNA strands, one at 5.2 kb which represent the expected band of the linearized pET-28 backbone and another band at 1.7 kb, which represent the kIspS gene (Additional file 1: Figure S1).
An expected band of 49 kDa was observed [9], corresponding to the binding of 125I-Cry2Ab to BBMV from susceptible insects (Fig. 1A, lane 2).
In addition to the expected band of the cytoplasmic receptor, a second band migrating at the approximate molecular weight of 40 kDa can be detected.
Conducting RT PCR and Western blot, we found the expected bands of FSHR in human and mouse adipose tissues (Fig. 2A, C); we also found the expected bands of FSHR in 3T3-L1 mouse preadipocyte cell line (Fig. 2B, C).
Two independent AS5 overexpression (AS5ox) lines were confirmed (AS5ox-1 and AS5ox-2), with expected bands of 2.3 and 0.9 kb.
As shown in Fig. 2D, the Southern blot resulted in the two expected bands of 6.9 kb (SacI– ClaI) and 10.3 kb (MluNI– ClaI) for type strain PG1.
As expected, bands of around 14, 30, and 76 kDa were observed for KMP-11r, TcHSP-70, and TrHSP-70 recombinant antigens, respectively.
Furthermore, when the plasmid was previously methylated by mHaeIII, the creation of the new site, because of modification of the sequence 5'-GGCCAG-3' to 5'-GGCTAG-3', was confirmed by gel detection of the two expected bands of 285 and 98 bp obtained after cutting.
