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In the production mode, we observed that most of the pools yielded >20 Mbp, more than half of the length expected, assembled in contigs greater than 4,000 bp in length.
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For all six of the constructs, four-of-four of the clones contained the expected fragments assembled in the predefined orientation, and had the expected sequence (data not shown).
Transcriptome redundancy is expected in assembled contigs due to the heuristic nature of the assembly process and the settings used to avoid assembly of slightly different sequences.
As expected, the assembled structures were disrupted by urea as the fluorescence intensity dramatically decreased.
As expected, the assembled ORFs belong all to the plant Hop family which exhibits a large amount of divergence with respect to the outgroup (Human Hop).
Thus, just as proteins preferentially fold via a limited number of energetically favorable folding pathways (Lindorff-Larsen et al., 2011), protein complexes should be expected to assemble via ordered assembly pathways.
A few conservative Republicans were pressing for a balanced budget, but House Republicans are expected to assemble a budget plan that closely resembles Mr. Bush's.
The department is expected to assemble as many as 10 prosecutors to work under Ms. Caldwell on the Enron investigation, which is likely to last many months.
Two hundred thousand women are expected to assemble in front of the Capitol, on January 21st, the day after the Inauguration, for the Women's March on Washington.
Several financiers have turned down the foundation job, and downtown officials are now expected to assemble a group of people to share it.
Mikyl Cordova, a spokeswoman for Madison Square Garden, said that the promoters expected to assemble a lineup of similar drawing power for the December concert.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com