Sentence examples for expected alternative splicing from inspiring English sources
(B ) RT-PCR analysis confirming the dramatic decrease of WT Sirt2 mature mRNA and the expected alternative splicing variants (SB) as the result of injecting Sirt2-MO.
(B ) RT-PCR analysis confirming the dramatic decrease of WT Myca mature mRNA and the expected alternative splicing variants (SB) as the result of injecting Myca-MO.
Here, we demonstrate by RT-PCR that, as expected, alternative splicing of exons 13, 14 and 16 takes place in human.
Most human genes are expected to have alternative splicing isoforms [ 19].
Isogroups are defined in the GS Assembler as a collection of contigs containing reads that imply connections between them, and are expected to include alternative splicing variants as well as homoeologs (A, B and D copies) and close paralogs.
Therefore alternative splicing is expected to produce proteins with altered properties.
Besides the expected FL mRNA, a CAIX alternative splicing isoform was detected in normal and in cancer cells, independently of the levels of hypoxia (Barathova et al, 2008).
Because sex-biased expression, and perhaps alternative splicing, is expected to be regulated by the canonical sex determination pathway, we addressed the level of control of tango13 within the sex determination network by examining its splicing via qPCR in XX females, XX pseudomales, and XY males.
If sex-differential isoform abundance is due to alternative splicing, we expect to find sex-differences in internal cassette and 3' exons, whereas if the difference is due to alternative promoter usage, we would expect to find differences in 5' exons.
If different introns have different requirements for PUF60 and U2AF65, then depleting U2AF65 in cells might be expected to have different effects on alternative splicing than PUF60 depletion.
Although this limitation should not reduce the gene prediction accuracy of our system in genomes with a low prevalence of alternative splicing, it is expected to affect the global performance in the case of genomes from organisms in which alternative splicing is a widespread phenomenon.
