Exact(1)
The default BLAST Expectation thresholds used were lowered to 100 to show weaker matches.
Similar(59)
We applied a strict expectation threshold of e = 0.01.
WU-BLAST (version 2.0MP-WashU) was run with the default BLASTP settings: matrix = BLOSUM62, Expectation Threshold = 10, ctxfactor = 1.0, no filtering.
To reduce the number of false predictions, the maximum expectation threshold was set to a stringent value of 2.0.
The nature of the largely positive responses gained may in part be influenced by a general sense of modesty in Ethiopia, the political environment, and low expectation threshold.
We used HMMER [ 17, 18] to identify Pfam domains in our gene models with an expectation threshold of E-value< 0.1 and compared the distribution to other sequenced and annotated ascomycetes.
Blast parameters used (WUBlast2.0): B 120 maximum number of database sequences for which any alignments will be reported E 1E-15 thexpectationon threshold for reporting database hits W 11 seed word length for the ungapped BLAST algorithm.
Second, for a more confident assessment of whether the assembled sequences were shared with insects, they were compared to protein sequences archived in the NCBI UniGene sets for Bombyx mori (build #7), Apis mellifera (build #5), Anopheles gambiae (build #29) and Drosophila melanogaster (build #37) using tBlastx with an expectation threshold set at E < 0.005.
Single nucleotide polymorphisms with a rate of missing genotypes > 5%, that did not conform Hardy-Weinberg expectations (threshold set at a P-value = 0.001) or that had a minor allele frequency below 0.05 were eliminated from the dataset.
From this set, different analyses were performed using more-stringent expectation value thresholds.
Expectation value thresholds for peptide identification were modified to maintain a false discovery rate <1% based on the number of spectra matching to reverse decoy sequences.
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