Exact(7)
We applied a strict expectation threshold of e = 0.01.
WU-BLAST (version 2.0MP-WashU) was run with the default BLASTP settings: matrix = BLOSUM62, Expectation Threshold = 10, ctxfactor = 1.0, no filtering.
To reduce the number of false predictions, the maximum expectation threshold was set to a stringent value of 2.0.
The nature of the largely positive responses gained may in part be influenced by a general sense of modesty in Ethiopia, the political environment, and low expectation threshold.
Blast parameters used (WUBlast2.0): B 120 maximum number of database sequences for which any alignments will be reported E 1E-15 thexpectationon threshold for reporting database hits W 11 seed word length for the ungapped BLAST algorithm.
We used HMMER [ 17, 18] to identify Pfam domains in our gene models with an expectation threshold of E-value< 0.1 and compared the distribution to other sequenced and annotated ascomycetes.
Similar(53)
The default BLAST Expectation thresholds used were lowered to 100 to show weaker matches.
Single nucleotide polymorphisms with a rate of missing genotypes > 5%, that did not conform Hardy-Weinberg expectations (threshold set at a P-value = 0.001) or that had a minor allele frequency below 0.05 were eliminated from the dataset.
The expectation value threshold (E-value: parameter –e) and inclusion threshold (parameter –h) were both set to 0.001.
Three PSI-BLAST iterations were conducted with an expectation value threshold set such that all previously identified sequences were recovered.
The CDD default E-value (expectation value) threshold of 0.01 was used in all experiments.
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