We expected to observe changes in the PAG given its involvement in descending modulation during migraine attacks [3].
Thus, if the selected promoter elements are involved in RGL2-mediated transcriptional regulation, we expected to observe changes in GFP-signal intensity; upon DEX-treatment, GFP-signal should increase compared to that observed in MOCK-treated transfected protoplasts, whereas protoplasts treated with DEX plus GA3 should not exhibit different intensities of GFP.
An issue that requires more thorough investigation is the presence of genes, e.g. repressors, for which it is expected to observe change in expression that contradicts the up- or down-regulated state of the pathway they are members of.
As the Apc-Cdkn1a network represents the intersection of signaling pathways emanating from Apc and from Cdkn1a, we expect to observe functional changes in network-associated proteins in response to perturbations at either Apc or Cdkn1a.
In addition, we expect to observe significant changes in metabolic, psychological and behavioural parameters 3 and 9 months following the intervention.
In addition, we expect to observe significant changes in metabolic, psychological and behavioural parameters following the intervention in both ethnic groups, resulting in reduced risk of developing T2DM.
Because our highest dose of AMM (8.73 mg/kg bw/day) contains only 25% ATR (1.79 mg/kg bw/day), we did not expect to observe a change in the timing of puberty.
As all hybrids were obtained from inbred parental lines and no parental fragment disappearance was not caused by chromosome elimination in the previously published studies [ 36, 37], every independently made F1 hybrid had the same phenotypic and band patterns, one would not expect to observe much change according to conventional theory.
It would therefore have been of interest to also assess gene expression earlier in the loading protocol (24 or 48 hours post stimulation or after the 1 Dose of load) where we would expect to observe more pronounced changes at the gene level, and this is a limitation of the current study.
Therefore, if DV or DE is associated with change in DC, then we expect to observe a big change in the distribution of r in the four 200-gene subsets in each human disease dataset.
