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India is due to ask the UK and other rich nations to share breakthroughs in renewable energy and other "clean" technology, and for help financing a huge expansion in efficiency and solar and wind power.
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In the second part of our work we have demonstrated that decrease in efficiency of expansion could be markedly improved by culturing of BM-MMSC under moderate hypoxic conditions and substantial decrease in cell seeding density.
After secondary expansion, the mutant cells gave rise to neurospheres in efficiency and of size ranges similar to those of control cells.
The small model system investigated in this study is easily amenable to expansion, and the deconvolution protocol would be expected to increase further in efficiency with larger systems.
To obtain enough cell for clinical use, the MSCs modified at a low efficiency need extensive expansion in vitro.
Furthermore, there are data, demonstrating that clonal seeding in combination with strong hypoxia (pO2=1%) results in increase of efficiency of MMSC expansion in vitro due to prevention of early onset of replicative senescence [ 54].
The expansion efficiency seen in vitro for 22-repeat substrates is in line with in vivo results.
We explore the individual role of supply-side measures (including cropland output intensification and cropland expansion), efficiency measures in the livestock system (increases in feed-conversion ratios) and demand-side measures (quantitative and qualitative changes in the human diet).
The money in the bill is enough to pay for a tremendous expansion of efficiency efforts across the country.
Such changes could save the industry billions of dollars in upgrades that utility officials said would otherwise hamper their expansion and efficiency.
But to use Notch ligands in HSC therapies of human diseases, efforts are required to improve ex vivo expansion efficiency and in vivo transplant engraftment.
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