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After isolation and expansion, cells were identified as adherent mesenchymal cells with substantial proliferation potential in vitro, and were also characterized by flow cytometry.
For cell line expansion, cells were trypsinized at 80% of confluency.
To measure the proliferative capacity of preadipocytes during the mitotic clonal expansion cells were counted at day 0 and day 3 of the differentiation process using a hemocytometer.
After 10 days of in vitro expansion, cells were harvested to test IFN-γ production following the re-stimulation of the cognate peptides (p65 for HLA-DR1, p57 and p69 for HLA-DP4 subjects).
To exclude the effect of dead cells due to polyL expansion, cells transfected with pQBI25-L32 and pQBI25-L24 were monitored for only 24 and 48 h, respectively (Figure 3).
Following expansion, cells were stimulated with 10 ng/ml doxycycline and 72 hours later media was collected and cell lysates were prepared to determine basal and induced EPO and rtTA expression levels respectively.
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Previous studies have been reported that cell division cycle protein 48 (CDC48; spots LU2 and LU3) may be directly involved in cell expansion, cell division and cell proliferation (Park et al. 2008; Rancour et al. 2004).
Cell expansion, cell division, and multiple developmental processes depend on BR.
Wnt pathway promotes cell proliferation, tissue expansion, cell fate determination, and terminal differentiation.
The transition from cell proliferation to expansion (cell growth without cell division) is controlled by a network of factors.
Small leaf size of exo could be based on impaired cell expansion, cell proliferation, or a combination of both.
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