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For each experiment we estimated the position of the leading edge of the expanding colony, the cell density profile along a transect throughout the entire expanding colony as well as measuring the degree of cell to cell clustering within the colony.
Yellow lines: front of the expanding colony.
(C ) Time-lapse of de novo occurring P − green clone (orange) within an expanding colony.
Here, glycosylated (wt) bacteria segregated at the front of the expanding colony.
The bacteria with glycosylated pili dominate the front of the expanding colony.
This allowed us to identify the location of individual cells within the expanding colony [ 7].
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For C = 0.4 0.5%, expanding colonies do not span the depth of the agar and develop pronounced front instabilities.
New chimneys appear near the edges of expanding colonies, at points where the speed of the outflow is already high, and do not change position if the water flow changes.
The expanding colonies of pluripotent ESCs then have the potential to develop into any cell type of the body.
Rather, it was performed by the conventional method of isolating and expanding colonies that are resistant to p53-mediated growth arrest.
Time course experiments showed that increases in the levels of ABCG2 during colony expansion were followed by a decrease in PPIX levels (Fig. 2N), indicating that ABCG2 might play a role in porphyrin homoeostasis in expanding colonies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com