However, as I think about integration opportunities, I am excited for the expanded toolbox we have to play with.
Finally, while there is much to do in the development of probes themselves, dissecting detailed mechanistic questions would benefit immensely from an expanded toolbox for manipulating metal ion fluxes in cells, such as a repertoire of specific metal chelators and photoactive caged metal compounds.
In this regard, a rapidly expanding toolbox of complement therapeutics is being developed to address unmet clinical needs in several immune-mediated and inflammatory diseases.
Meanwhile, whereas gene expression systems for metabolic engineering have traditionally been static or externally tunable, an expanding toolbox of metabolite-responsive gene regulators coupled with deeper insights into metabolic bottlenecks have given rise to dynamic control over engineered pathways.
More studies are needed, utilizing an expanding toolbox of specific autophagy inhibitors, to parse out specific IFN-controlled signaling outcomes for autophagy in different cell types.
With an expanding "toolbox" of comprehensive basic, medical, surgical, and technological approaches, it is expected that these novel findings will soon be translated to the clinical practice.
New hypotheses can now be addressed experimentally by exploiting the expanding toolbox of reverse genetics, such as insertion mutants combined with targeted metabolic profiling, and by reverse biochemistry using collections of recombinant proteins and medium throughput screening of substrate collections [ 58].
Recent research has greatly expanded our toolbox for plastid genome engineering and many new proof-of-principle applications have highlighted the enormous potential of the transplastomic technology in both crop improvement and the development of plants as bioreactors for the sustainable and cost-effective production of biopharmaceuticals, enzymes and raw materials for the chemical industry.
Methods of unnatural amino acid mutagenesis have further expanded the toolbox for bioinorganic chemists.
Oxidative labeling of peptides and proteins has expanded the toolbox of biochemists and notable progress has been made in the past decade.
A wide set of techniques to produce hydroxyl radicals have expanded the toolbox available for footprinting experiments (see " Production and use of oxidizing agents").
