Sentence examples for expanded template from inspiring English sources

He and his brother Charlie incorporated those influences, along with soul, funk and blues elements, into a new ensemble called Harlem River Drive, creating the expanded template that marked most of his best work throughout the 1970s, like "Vamonos Pa'l Monte" and "Adoración".

Herein, we present a simple scheme to expand template stripping beyond the coinage metals to obtain structures for a broader set of applications.

Our second goal was to use these markers to generate a high density linkage map, with paired-end sequences [ 40] used to expand template length to annotate as many SNPs as possible.

The product of this procedure was PCR amplified (using primers binding to the previously added linkers) with the Long Expand Template PCR System Kit (Roche), and PCR products were ligated to the pSTC1.3 no-background vector (StabyCloning™ kit, Delphi Genetics) and transformed into competent cells (Delphi Genetics).

Long Expand Template PCR System kit (Roche Diagnostics, Inc).

The coding sequences were amplified with the Long Expand Template PCR-System (Roche, Mannheim, Germany).

The 16.5 kb mitochondrial genome was amplified in two fragments using the Expand Template Long PCR Kit from Roche Diagnostics (Mannheim, Germany) according to the manufacturer's protocol.

Implementing and expanding that template, Ann has transformed an interest in giving into a career as philanthropist.

Using the expand long Template PCR system (Roche), the genomic region of γCOP and a ∼5.8 kb region between γCOP and the proximal gene CG1499 (ending at a SalI site 3' of the CG1499 gene) was amplified from ry506 flies according to the manufacturer's cycling conditions, using primer 100 for the 5' end and primer 101 for the 3'end.

In brief, 2 μl of digested RCA products were used for amplification in a 50-μl reaction together with 38.75 μl DEPC-treated water, 2.5 μl dNTP mix (10 mM each)), 0.5 μl of each forward and reverse primers (20 μM), 5 μL of 10x PCR buffer II with MgCl2, and 0.75 μl of the expand long template enzyme mix which contains high-fidelity thermostable Tgo DNA polymerase with proofreading activity.

This library also gives the possibility to expand optimization templates.