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Despite this potential advantage, it remains to be seen if these theoretical predictions result in a measurable clinical effect that outweighs the added complexity of the expanded stimulation parameter search space generated by the more flexible technology.
Similar(59)
Altogether, these results provide an example that antigen-specific CD8+ T cell populations of high or low frequency in the periphery can efficiently be expanded by stimulation with long-term CD40-stimulated B cells, and acquire or maintain antigen-specific cytolytic function after expansion.
Pharmacological Magnetic Resonance Imaging (phMRI) methods have significantly expanded the stimulation repertoire available to preclinical fMRI research, by allowing to selectively probe the activity of specific brain circuitries and neurotransmitter systems.
In vitro stimulation of peripheral blood mononuclear cells (PBMC) from two immunocompetent HLA-A*02/HLA-B*07 HLA-A*02/HLA-B*07 HLA-A*02/HLA-B*07c T lymphocyte (CTL) precursors specindividualsth HLA-A*02 and HLA-B*07 restrindicatedthats were present and cytotoxicexpanded by sTimulymphocyteh the CTLnate precursors
In these patients, overall we identified 26 rearrangements expanded by stimulation with huCollp261 273 but not with huCollp289 303.
Remarkably, synovial fluid NKT cells of both responders and nonresponders expanded upon stimulation, indicating that the impaired NKT cell function in nonresponders is restricted to the blood compartment.
CD4+ cells- cells isolated from patients' PBMCs were expanded by stimulation with anti-CD3 and CD28, TGFβ and RA, then sorted into CD25+ and CD25- populations.
Nevertheless, their frequency could be significantly expanded after stimulation in vitro with Chlamydia-infected autologous DCs, suggesting that these cells have full replicative capacity.
However, NKT cells isolated from synovial fluid from both responders and nonresponders expanded upon stimulation with α-galactosylceramide. Analysis of the cytokine profile of CD4+ and CD4- PBMC derived NKT cell lines from RA patients revealed a significantly reduced number of IL-4 producing cells.
A coculture system using rat cardiomyocytes had been effective in inducing a cardiomyocyte-like phenotype in CD133+ hematopoietic progenitors that were first selected from UCB by immunomagnetic separation and then expanded by stimulation with platelet-derived and epidermal growth factors [ 54].
A standard phase encoding expanding ring stimulation paradigm was used to map the retinotopic (eccentricity) organization in areas V2 and V3.
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Justyna Jupowicz-Kozak
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