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BiFC can be expanded in combination with BRET (Gandia et al., 2008) or in multicolour assays (Mervine et al., 2006) to examine co-operative or competitive multi-component interactions.
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As shown in Figure 2b, injection of expanded Tregs in combination with tumor cells and bsAb did not reduce tumor outgrowth in mice, substantiating that Tregs are not capable of eliminating co-injected cancer cells.
We have now expanded on this point in combination with our response to the point below.
The expanded DGT equation (eq 5) in combination with the lateral diffusion correction factor kLD with or without kDBL resulted in the best cb estimates for simulated and experimental data.
The results demonstrated that expanded sorted CD4+ T-helper lymphocytes from the tumor-draining lymph node have therapeutic efficacy on their own, and a synergistic effect was found when CD4+ T-helper lymphocytes were used in combination with expanded cytotoxic CD8+ T-lymphocytes.
This was a single-arm open-label expanded access study of lapatinib in combination with capecitabine for male and female patients with HER2 overexpressing locally advanced breast cancer (LABC) defined as stage IIIb/c with a T4 lesion or metastatic (stage IV) disease.
In view of the expanding use of PET cameras in combination with new elegant radiofluorination techniques, it is to be expected that new, improved F-based bombesins for oncological PET applications will soon appear.
The first example of clinical cartilage tissue engineering was performed in 1987 when a knee with an articular cartilage defect on the femoral condyle was treated by implanting the patient's own chondrocytes that had been expanded in vitro into the defect in combination with a covering mechanical membrane the periosteum.
The toxicity of V γ9V δ2 T cells expanded with zoledronate and adoptively transferred in combination with a zoledronate infusion is yet to be reported in solid tumour patients.
In the clinical study described here, the safety and feasibility of adoptive transfer of large numbers of ex vivo expanded autologous V γ9V δ2 T cells in combination with zoledronate infusion was investigated in patients with solid tumours.
These expanded NK cells will be tested in combination with bortezomib, an FDA approved proteasome inhibitor that has been shown to sensitize tumor cells to TRAIL-dependent NK cell lysis.
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